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KMID : 0365819660060010103
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Journal of Pusan Medical College
1966 Volume.6 No. 1 p.103 ~ p.116
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Effects of Hypothermia on Several Physiologic Functions and on Preservation of Isolated Liver in the Dog
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Abstract
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The body temperature was lowered by surface cooling to 28?0.5?C. in 10 anesthetized dogs and several physiologic functions were studied during tie course of hypot}ermia.At tie end of hypothermic experiments, tie liver ´, as isolated and was immediately perfused with 4`C. saline and tie organ was r. a:nta_ned at about 4?C. in saline solution for a period of 64 hours. Subsequently, a portion of tie liver tissue was taken for histo´ogical studies. The results may be summarized as follows
1. In lowering the rectal temperature to 28:1 0. 5IS?C. with the present method, it took on tic average 5 77 mh.per Kg. of body weight. The ventricular fibrillaton and adrift in body temperature were oLseived in one dog respectively.
2. The heart rate and the blood p-essures (arterial and vencus)increased slightly during tie initial phase of hypothermia after which they decreased progressive´y in pa´allel to the reduction rif body temperature. Similar trends were also observed in the portal venous pressure.
3. The hematocrit ratio increased hom 51?3.4% before hypothermia to 57±18% at the end of hypothermic period. The arterial 02 content also increased significantly, while the arterial CO2content was invariably lowered in hypothermia.
4. There were little autolytic changcs in the isolated liver perfused with 4 C. saline and maintained at about 4?C. for a period of 4 hours after which histologic signs of autolysis appeared. In contrast, the liver isolated at no mal body temperature and perfused with 29?C. saline shoved signs of autolysis within 60 minutes. These results indicate that one can preserve the liver up to 4 hours by isolating it from previously induced hypothermic dogs and then by perfusing it with cold saline and ma´n´a´ring at about 4?C. The implication of this finding is discussed in the light of clinical application.
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KEYWORD
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